Dynamic Light Scattering (DLS) – AvidNano W130i

Location: Lab 0.5
Responsible: Rita Branquinho
Description: Dynamic Light Scattering (DLS) is one of the most popular light scattering techniques because it allows particle sizing down to 1 nm diameter. Typical applications are emulsions, micelles, polymers, proteins, nanoparticles or colloids.

Measurement range

(hydrodynamic diameter)


1 – 1000 nm

Molecular weight range

192 Da (e.g., caffeine) — 25 MDa

Min sample concentration

0.1 mg/mL of lysozyme in phosphate buffer (1.9 nm radius), much lower concentration required for larger particles

Sample cuvette types (typical)

• Blade cell, 5 µL polycarbonate disposable 

• Generic 12.5 mm square-walled cuvettes, 4 clear windows

Temperature control range


0–90 °C

Temperature control accuracy


±0.2 °C

Light source

660 nm laser diode

Detector type

Photodiode module

Environmental conditions

Temperature range: 10–40 °C