Dynamic Light Scattering (DLS) – AvidNano W130i

Location: Nanofabrication Lab
Responsible: Rita Branquinho
Description: Dynamic Light Scattering (DLS) is one of the most popular light scattering techniques because it allows particle sizing down to 1 nm diameter. Typical applications are emulsions, micelles, polymers, proteins, nanoparticles or colloids.

Measurement range (hydrodynamic diameter): 1 – 1000 nm
Molecular weight range: 192 Da (e.g., caffeine) — 25 MDa
Min sample concentration: 0.1 mg/mL of lysozyme in phosphate buffer (1.9 nm radius), much lower concentration required for larger particles
Sample cuvette types (typical):
• Blade cell, 5 µL polycarbonate disposable
• Generic 12.5 mm square-walled cuvettes, 4 clear windows
Temperature control range: 0–90 °C
Temperature control accuracy: ±0.2 °C
Light source: 660 nm laser diode
Detector type: Photodiode module
Environmental conditions:Temperature range: 10–40 °C