Enhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers

TitleEnhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers
Publication TypeJournal Article
Year of Publication2016
AuthorsGuler Z, Silva JC, Sarac AS
JournalJournal of Biomaterials Applications
KeywordsBiofunctionalization, Bone, Bone morphogenetic protein-2, Electrospun nanofibers, In-vitro, Nanofibers, Phosphatases, Poly (m -antranilic acid), Proteins, Scaffolds (biology), Tissue engineering

Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2. © SAGE Publications.