@article {Guler2016743,
	title = {Enhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers},
	journal = {Journal of Biomaterials Applications},
	volume = {31},
	number = {5},
	year = {2016},
	note = {cited By 0},
	pages = {743-754},
	publisher = {SAGE Publications Ltd},
	abstract = {Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2. {\textcopyright} SAGE Publications.},
	keywords = {Biofunctionalization, Bone, Bone morphogenetic protein-2, Electrospun nanofibers, In-vitro, Nanofibers, Phosphatases, Poly (m -antranilic acid), Proteins, Scaffolds (biology), Tissue engineering},
	issn = {08853282},
	doi = {10.1177/0885328216660379},
	url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994102233\&doi=10.1177\%2f0885328216660379\&partnerID=40\&md5=20f6a93639532ab51063cda593c17504},
	author = {Guler, Z. and Silva, J.C. and Sarac, A.S.}
}
