Charged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation

TitleCharged pullulan derivatives for the development of nanocarriers by polyelectrolyte complexation
Publication TypeJournal Article
Year of Publication2016
AuthorsDionísio M a, c d Braz L b, Corvo M e, f g Lourenço JP b, f Grenha A a, f Rosa da Costa AM b
JournalInternational Journal of Biological Macromolecules
KeywordsArticle, bovine serum albumin, carbohydrate analysis, carrageenan, chemical modification, Chitosan, complex formation, controlled study, cytotoxicity, hydrogen bond, immobilization, in vitro study, n, n dimethylformamide, nanocarrier, nanoparticle, particle size, polyelectrolyte, polysaccharide, pullulan, pullulan derivative, quaternary ammonium derivative, small interfering RNA, sorbitan oleate, sulfur oxide, synthesis, unclassified drug

Pullulan, a neutral polysaccharide, was chemically modified in order to obtain two charged derivatives: reaction with SO3.DMF complex afforded a sulfate derivative (SP), while reaction with glycidyltrimethylammonium chloride gave a quaternary ammonium salt (AP). The presence of the charged groups was confirmed by FTIR. Assessment of the positions where the reaction took place was based on 1H- and 13C NMR (COSY, HSQC-TOCSY, HSQC-DEPT, and HMBC) experiments. Estimation of the degree of substitution (DS) was made from elemental analysis data, and further confirmed by NMR peak areas in the case of AP. These new derivatives showed the capability to condense with each other, forming nanoparticles with the ability to associate a model protein (BSA) and displaying adequate size for drug delivery applications, therefore making them good candidates for the production of pullulan-based nanocarriers by polyelectrolyte complexation. © 2016 Elsevier B.V.