<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Martins, G.V.</style></author><author><style face="normal" font="default" size="100%">Marques, A.C.</style></author><author><style face="normal" font="default" size="100%">Fortunato, E.</style></author><author><style face="normal" font="default" size="100%">Sales, M.G.F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">8-hydroxy-2′-deoxyguanosine (8-OHdG) biomarker detection down to picoMolar level on a plastic antibody film</style></title><secondary-title><style face="normal" font="default" size="100%">Biosensors and Bioelectronics</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Antibodies</style></keyword><keyword><style  face="normal" font="default" size="100%">Bioassay</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomarkers</style></keyword><keyword><style  face="normal" font="default" size="100%">Biomolecules</style></keyword><keyword><style  face="normal" font="default" size="100%">Biosensors</style></keyword><keyword><style  face="normal" font="default" size="100%">Body fluids</style></keyword><keyword><style  face="normal" font="default" size="100%">Chemical detection</style></keyword><keyword><style  face="normal" font="default" size="100%">Confocal microscopy imaging</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclic voltammetry</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrochemical biosensor</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrochemical impedance spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrodes</style></keyword><keyword><style  face="normal" font="default" size="100%">Electropolymerization</style></keyword><keyword><style  face="normal" font="default" size="100%">Experimental parameters</style></keyword><keyword><style  face="normal" font="default" size="100%">Fluorescein isothiocyanate</style></keyword><keyword><style  face="normal" font="default" size="100%">Fourier transform infrared spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">In-situ construction</style></keyword><keyword><style  face="normal" font="default" size="100%">Initial concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Label-free biosensor</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecularly Imprinted Polymer</style></keyword><keyword><style  face="normal" font="default" size="100%">Monomers</style></keyword><keyword><style  face="normal" font="default" size="100%">Thin films</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-84976863902&amp;doi=10.1016%2fj.bios.2016.06.052&amp;partnerID=40&amp;md5=07409fec5086d52b947ccede4ceda6e5</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Elsevier Ltd</style></publisher><volume><style face="normal" font="default" size="100%">86</style></volume><pages><style face="normal" font="default" size="100%">225-234</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An innovative biosensor assembly relying on a simple and straightforward in-situ construction is presented to monitor urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) down to the pmol/L level. The sensing film of the biosensor consisted of a molecularly imprinted polymer (MIP) layer for 8-OHdG assembled on a gold electrode through electropolymerization of monomer combined with the template. The analytical features of the resulting biosensor were assessed by Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). Some experimental parameters such as the initial concentration of the monomer and the ratio template-monomer were investigated and optimized in order to finely tune the performance of the MIP-based sensor. Under optimal conditions, the developed biosensor was able to rebind 8-OHdG with a linear response against EIS from 0.1 to 100 pg/ml 3.5-3500 pM. The interference of coexisting species was tested, also with calibrations on urine samples, and good selectivity towards 8-OHdG was obtained. RAMAN spectroscopy, FTIR and SEM evaluations of the prepared films confirmed the formation of a polyphenol thin-film on the electrode surface. The presence and distribution of the imprinted cavities on the MIP layer was confirmed by confocal microscopy imaging of the film, after a post-treatment with Fluorescein Isothiocyanate (FITC) labeled 8-OHdG antibody. Overall, this label-free biosensor for urinary 8-OHdG detection constitutes a promising low-cost alternative to the conventional immunoassay approaches, due to its simplicity, stability, high sensitivity and selectivity for biological sample assays, opening new doors for other applications. © 2016</style></abstract><notes><style face="normal" font="default" size="100%">cited By 0</style></notes></record></records></xml>