<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Santos, T.a</style></author><author><style face="normal" font="default" size="100%">Carvalho, J.a</style></author><author><style face="normal" font="default" size="100%">Corvo, M.C.b c</style></author><author><style face="normal" font="default" size="100%">Cabrita, E.J.b</style></author><author><style face="normal" font="default" size="100%">Queiroz, J.A.a</style></author><author><style face="normal" font="default" size="100%">Cruz, C.a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">L-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Biological Macromolecules</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">4 (2 hydroxyethyl) 1 piperazineethanesulfonic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">agar gel electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">arginine</style></keyword><keyword><style  face="normal" font="default" size="100%">Article</style></keyword><keyword><style  face="normal" font="default" size="100%">biosensor</style></keyword><keyword><style  face="normal" font="default" size="100%">chemical structure</style></keyword><keyword><style  face="normal" font="default" size="100%">deprotection reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">dipeptide derivative</style></keyword><keyword><style  face="normal" font="default" size="100%">dissociation constant</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA purification</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA supercoiling</style></keyword><keyword><style  face="normal" font="default" size="100%">epitope mapping</style></keyword><keyword><style  face="normal" font="default" size="100%">equilibrium constant</style></keyword><keyword><style  face="normal" font="default" size="100%">hydrophobicity</style></keyword><keyword><style  face="normal" font="default" size="100%">immobilization</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Nuclear magnetic resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">nuclear Overhauser effect</style></keyword><keyword><style  face="normal" font="default" size="100%">plasmid</style></keyword><keyword><style  face="normal" font="default" size="100%">proton nuclear magnetic resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">separation technique</style></keyword><keyword><style  face="normal" font="default" size="100%">sepharose</style></keyword><keyword><style  face="normal" font="default" size="100%">Surface plasmon resonance</style></keyword><keyword><style  face="normal" font="default" size="100%">synthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">tryptophan</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://www.scopus.com/inward/record.uri?eid=2-s2.0-84960079870&amp;doi=10.1016%2fj.ijbiomac.2016.02.079&amp;partnerID=40&amp;md5=265e4e461bfc8ee90dc50a1bec45dcb9</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Elsevier</style></publisher><volume><style face="normal" font="default" size="100%">87</style></volume><pages><style face="normal" font="default" size="100%">385-396</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The present study focus on the preparation of chromatography supports for affinity-based chromatography of supercoiled plasmid purification. Three l-tryptophan based supports are prepared through immobilization on epoxy-activated Sepharose and characterized by HR-MAS NMR. The SPR is employed for a fast screening of l-tryptophan derivatives, as potential ligands for the biorecognition of supercoiled isoform, as well as, to establish the suitable experimental conditions for the chromatography. The results reveal that the overall affinity is high (KD = 10-9 and 10-8 M) and the conditions tested show that the use of HEPES 100 mM enables the separation and purification of supercoiled at T = 10 °C. The STD-NMR is performed to accomplish the epitope mapping of the 5'-mononucleotides bound to l-tryptophan derivatives supports. The data shows that the interactions between the three supports and the 5'-mononucleotides are mainly hydrophobic and π-π stacking. The chromatography experiments are performed with l-tryptophan support and plasmids pVAX-LacZ and pPH600. The supercoiled isoform separation is achieved at T = 10 °C by decreasing the concentration of (NH4)2SO4 from 2.7 to 0 M in HEPES for pVAX-LacZ and 2.65 M to 0 M in HEPES for pPH600.Overall, l-tryptophan derivatives can be a promising strategy to purify supercoiled for pharmaceutical applications. © 2016 Elsevier B.V.</style></abstract><notes><style face="normal" font="default" size="100%">cited By 0</style></notes></record></records></xml>