%0 Journal Article %J Journal of Biomaterials Applications %D 2016 %T Enhanced osteogenesis on biofunctionalized poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers %A Guler, Z. %A Silva, J.C. %A Sarac, A.S. %I SAGE Publications Ltd %K Biofunctionalization %K Bone %K Bone morphogenetic protein-2 %K Electrospun nanofibers %K In-vitro %K Nanofibers %K Phosphatases %K Poly (m -antranilic acid) %K Proteins %K Scaffolds (biology) %K Tissue engineering %P 743-754 %R 10.1177/0885328216660379 %U https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994102233&doi=10.1177%2f0885328216660379&partnerID=40&md5=20f6a93639532ab51063cda593c17504 %V 31 %X Biofunctionalized nanofibers with a desired biological function can be used as a tissue engineering scaffold due to their small fiber diameters and porous structure. In the present study, poly(ϵ-caprolactone)/poly(m-anthranilic acid) nanofibers were biofunctionalized with covalent immobilization of bone morphogenetic protein-2 (BMP-2) through 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide activation. Fourier transform infrared analysis of the nanofiber surfaces confirmed the successful immobilization. The amount of immobilized BMP-2 was determined with bicinchoninic acid protein assay. The nanofibers before and after BMP-2 immobilization were non-cytotoxic and enhanced the attachment and proliferation of Saos-2 cells. Biofunctionalization of nanofibers with BMP-2 promoted in vitro osteogenic activity. The alkaline phosphatase activity and calcium mineralizatio of cells after 14 days of in vitro culture were enhanced on nanofibers with immobilized BMP-2. © SAGE Publications. %Z cited By 0